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pbluescript (pbs) iiks  (Agilent technologies)


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    Agilent technologies pbluescript (pbs) iiks
    Pbluescript (Pbs) Iiks, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbluescript (pbs) iiks/product/Agilent technologies
    Average 90 stars, based on 1 article reviews
    pbluescript (pbs) iiks - by Bioz Stars, 2026-03
    90/100 stars

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    Binding of RHA to circular DNA. (A) Increasing amounts (0.25, 0.5 and 1 µg) of RHA were incubated for 30 min at 37°C in reactions (20 µl) containing 0.25 µg of either linear (lanes 2 and 6–8) or <t>supercoiled</t> circular <t>pBS</t> (lanes 1 and 3–5). Subsequently, reaction mixtures were resolved on a 0.7% agarose gel in 1× TAE, and DNA was visualized by ethidium bromide staining. (B) Increasing amounts (0.125, 0.25, 0.5, 1 and 2 µg) of RHA were added in reactions containing 0.25 µg of supercoiled pBS and 8 ng of topoisomerase IIα. After a 30 min incubation at 37°C, topoisomers were resolved on a 0.7% agarose gel. (C) RHA (1 µg) and topoisomerase IIα (8 ng) or calf thymus topoisomerase I (1 U) were added in the indicated reaction containing 0.25 µg of supercoiled pBS. After a 30 min incubation at 37°C, topoisomers were analyzed as described in (B) and quantified using the QuantityOne software. scDNA, supercoiled DNA.
    Supercoiled Pbluescript Iiks – (Pbs), supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    pbluescript (pbs) iiks  (Agilent technologies)
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    Agilent technologies pbluescript (pbs) iiks
    Binding of RHA to circular DNA. (A) Increasing amounts (0.25, 0.5 and 1 µg) of RHA were incubated for 30 min at 37°C in reactions (20 µl) containing 0.25 µg of either linear (lanes 2 and 6–8) or <t>supercoiled</t> circular <t>pBS</t> (lanes 1 and 3–5). Subsequently, reaction mixtures were resolved on a 0.7% agarose gel in 1× TAE, and DNA was visualized by ethidium bromide staining. (B) Increasing amounts (0.125, 0.25, 0.5, 1 and 2 µg) of RHA were added in reactions containing 0.25 µg of supercoiled pBS and 8 ng of topoisomerase IIα. After a 30 min incubation at 37°C, topoisomers were resolved on a 0.7% agarose gel. (C) RHA (1 µg) and topoisomerase IIα (8 ng) or calf thymus topoisomerase I (1 U) were added in the indicated reaction containing 0.25 µg of supercoiled pBS. After a 30 min incubation at 37°C, topoisomers were analyzed as described in (B) and quantified using the QuantityOne software. scDNA, supercoiled DNA.
    Pbluescript (Pbs) Iiks, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbluescript (pbs) iiks/product/Agilent technologies
    Average 90 stars, based on 1 article reviews
    pbluescript (pbs) iiks - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

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    Binding of RHA to circular DNA. (A) Increasing amounts (0.25, 0.5 and 1 µg) of RHA were incubated for 30 min at 37°C in reactions (20 µl) containing 0.25 µg of either linear (lanes 2 and 6–8) or supercoiled circular pBS (lanes 1 and 3–5). Subsequently, reaction mixtures were resolved on a 0.7% agarose gel in 1× TAE, and DNA was visualized by ethidium bromide staining. (B) Increasing amounts (0.125, 0.25, 0.5, 1 and 2 µg) of RHA were added in reactions containing 0.25 µg of supercoiled pBS and 8 ng of topoisomerase IIα. After a 30 min incubation at 37°C, topoisomers were resolved on a 0.7% agarose gel. (C) RHA (1 µg) and topoisomerase IIα (8 ng) or calf thymus topoisomerase I (1 U) were added in the indicated reaction containing 0.25 µg of supercoiled pBS. After a 30 min incubation at 37°C, topoisomers were analyzed as described in (B) and quantified using the QuantityOne software. scDNA, supercoiled DNA.

    Journal:

    Article Title: RNA helicase A interacts with dsDNA and topoisomerase II?

    doi:

    Figure Lengend Snippet: Binding of RHA to circular DNA. (A) Increasing amounts (0.25, 0.5 and 1 µg) of RHA were incubated for 30 min at 37°C in reactions (20 µl) containing 0.25 µg of either linear (lanes 2 and 6–8) or supercoiled circular pBS (lanes 1 and 3–5). Subsequently, reaction mixtures were resolved on a 0.7% agarose gel in 1× TAE, and DNA was visualized by ethidium bromide staining. (B) Increasing amounts (0.125, 0.25, 0.5, 1 and 2 µg) of RHA were added in reactions containing 0.25 µg of supercoiled pBS and 8 ng of topoisomerase IIα. After a 30 min incubation at 37°C, topoisomers were resolved on a 0.7% agarose gel. (C) RHA (1 µg) and topoisomerase IIα (8 ng) or calf thymus topoisomerase I (1 U) were added in the indicated reaction containing 0.25 µg of supercoiled pBS. After a 30 min incubation at 37°C, topoisomers were analyzed as described in (B) and quantified using the QuantityOne software. scDNA, supercoiled DNA.

    Article Snippet: Supercoiled pBluescript IIKS – (pBS) was prepared using the Qiagen plasmid kit.

    Techniques: Binding Assay, Incubation, Agarose Gel Electrophoresis, Staining, Software